{"id":25258,"date":"2018-04-21T03:14:14","date_gmt":"2018-04-21T03:14:14","guid":{"rendered":"http:\/\/www.kurzweilai.net\/?p=313388"},"modified":"2018-04-24T03:49:38","modified_gmt":"2018-04-24T03:49:38","slug":"new-microscope-captures-awesome-animated-3d-movies-of-cells-at-high-resolution-and-speed","status":"publish","type":"post","link":"https:\/\/hoo.central12.com\/fugic\/2018\/04\/21\/new-microscope-captures-awesome-animated-3d-movies-of-cells-at-high-resolution-and-speed\/","title":{"rendered":"New microscope captures awesome animated 3D movies of cells at high resolution and speed"},"content":{"rendered":"<p><iframe frameborder=\"0\" height=\"315\" src=\"https:\/\/www.youtube.com\/embed\/Hz0VlUVjYfI?rel=0\" width=\"560\"><\/iframe><\/p>\n<p>HHMI Howard Hughes Medical Institute | An immune cell explores a zebrafish&#8217;s inner ear<\/p>\n<p>By combining two state-of-the-art imaging technologies, Howard Hughes Medical Institute Janelia Research Campus scientists, led by 2014 <a href=\"http:\/\/www.hhmi.org\/news\/eric-betzig-wins-2014-nobel-prize-chemistry\" >chemistry Nobel laureate<\/a> physicist\u00a0<a href=\"https:\/\/www.janelia.org\/people\/eric-betzig\" >Eric Betzig<\/a>, have imaged living cells at unprecedented 3D detail and speed, the scientists report on April 19, 2018 in an <a href=\"http:\/\/science.sciencemag.org\/content\/360\/6386\/eaaq1392\" >open-access paper<\/a> in the journal\u00a0<em>Science<\/em>.<\/p>\n<p>In\u00a0stunning videos of animated worlds, cancer cells crawl, spinal nerve circuits rewire, and we travel down through the endomembrane mesh of a zebrafish eye.<\/p>\n<p><strong>Microscope flaws.<\/strong> The new adaptive optics\/lattice light sheet microscopy (AO-LLSM) system addresses two fundamental flaws with traditional microscopes. They&#8217;re too slow to study natural three-dimensional (3D) cellular processes in real time and in detail (the sharpest views have been limited to isolated cells immobilized on glass slides).<\/p>\n<p>And the bright light required for imaging causes photobleaching and other cellular damages. These microscopes bathe cells with light thousands to millions of times more intense than the desert sun, says Betzig &#8212; damaging or killing the organism being studied.<\/p>\n<p><strong>Merging adaptive optics and rapid scanning.<\/strong> To meet these challenges, Betzig and his team created a microscopy system that merges two technologies: Aberration-correcting adaptive-optics technology used by astronomers to provide clear views of distant celestial objects through Earth\u2019s turbulent atmosphere; and non-invasive lattice light sheet microscopy, which rapidly and repeatedly sweeps an ultra-thin sheet of light through the cell (avoiding light damage) while acquiring a series of 2D images and building a high-resolution 3D movie of subcellular dynamics.<\/p>\n<div id=\"attachment_313528\" class=\"wp-caption aligncenter\" style=\"width: 650px;  border: 1px solid #dddddd; background-color: #f3f3f3; padding-top: 4px; margin: 10px; text-align:center; display: block; margin-right: auto; margin-left: auto;\"><a href=\"http:\/\/www.kurzweilai.net\/new-microscope-captures-awesome-animated-3d-movies-of-cells-at-high-resolution-and-speed\/zebrafish-embryo-neural-circuit-development\" rel=\"attachment wp-att-313528\"><img class=\" wp-image-313528\" title=\"zebrafish embryo neural circuit development\" src=\"http:\/\/www.kurzweilai.net\/images\/zebrafish-embryo-neural-circuit-development.png\" alt=\"\" width=\"640\" height=\"275\" \/><\/a><p style=' padding: 0 4px 5px; margin: 0;'  class=\"wp-caption-text\">Zebrafish embryo spinal cord neural circuit development (credit: HHMI Howard Hughes Medical Institute)<\/p><\/div>\n<p>The combination allows for the study of 3D subcellular processes in their native multicellular environments at high spatiotemporal (space and time) resolution.<\/p>\n<p><strong>Desk version.<\/strong> Currently, the new microscope fills a 10-foot-long table. \u201cIt\u2019s a bit of a Frankenstein\u2019s monster right now,\u201d says Betzig. His team is working on a next-generation version that should fit on a small desk at a cost within the reach of individual labs. The first such instrument will go to Janelia\u2019s\u00a0<a href=\"https:\/\/www.janelia.org\/open-science\/advanced-imaging-center-aic\" >Advanced Imaging Center<\/a>, where scientists from around the world can apply to use it. Plans that scientists can use to create their own microscopes will also be made freely available.<\/p>\n<p>Ultimately, Betzig hopes that the adaptive optical version of the lattice microscope will be commercialized, as was the base lattice instrument before it. That could bring adaptive optics into the mainstream.<\/p>\n<p><strong><a href=\"https:\/\/vimeo.com\/album\/5037955\" >Movie Gallery: Lattice Light Sheet Microscopy with Adaptive Optics<\/a>\u00a0<\/strong><\/p>\n<p><a href=\"https:\/\/youtu.be\/VY_v6G_ZLzE?t=5\" ><img title=\"Endocytosis in a human stem cell derived organoid.\" src=\"https:\/\/www.hhmi.org\/sites\/default\/files\/news\/2018\/movie-1_243352-2_4.gif\" alt=\"\" data-delta=\"11\" data-fid=\"13177\" data-media-element=\"1\" \/><\/a><a href=\"https:\/\/youtu.be\/VY_v6G_ZLzE?t=5\" >Endocytosis in a human stem cell derived organoid.<\/a> <a href=\"https:\/\/www.youtube.com\/watch?v=VY_v6G_ZLzE&amp;feature=youtu.be&amp;t=55\" ><img title=\"Clathrin-mediated endocytosis in vivo.\" src=\"https:\/\/www.hhmi.org\/sites\/default\/files\/news\/2018\/movie-2_243352-3_1.gif\" alt=\"\" data-delta=\"12\" data-fid=\"13178\" data-media-element=\"1\" \/><\/a><a href=\"https:\/\/www.youtube.com\/watch?v=VY_v6G_ZLzE&amp;feature=youtu.be&amp;t=55\" >Clathrin-mediated endocytosis in vivo.<\/a> <a href=\"https:\/\/www.youtube.com\/watch?v=VY_v6G_ZLzE&amp;feature=youtu.be&amp;t=165\" ><img title=\"Clathrin localization in muscle fibers.\" src=\"https:\/\/www.hhmi.org\/sites\/default\/files\/news\/2018\/movie-3_243352-4_1.gif\" alt=\"\" data-delta=\"13\" data-fid=\"13179\" data-media-element=\"1\" \/><\/a><a href=\"https:\/\/www.youtube.com\/watch?v=VY_v6G_ZLzE&amp;feature=youtu.be&amp;t=165\" >Clathrin localization in muscle fibers.<\/a><\/p>\n<div><a href=\"https:\/\/www.youtube.com\/watch?v=VY_v6G_ZLzE&amp;feature=youtu.be&amp;t=360\" ><img title=\"Subcellular imaging of organelle dynamics in the early zebrafish brain.\" src=\"https:\/\/www.hhmi.org\/sites\/default\/files\/news\/2018\/movie-4_243352-5_1.gif\" alt=\"\" data-delta=\"14\" data-fid=\"13180\" data-media-element=\"1\" \/><\/a><a href=\"https:\/\/www.youtube.com\/watch?v=VY_v6G_ZLzE&amp;feature=youtu.be&amp;t=360\" >Organelle dynamics in the early zebrafish brain.<\/a> <a href=\"https:\/\/www.youtube.com\/watch?v=VY_v6G_ZLzE&amp;feature=youtu.be&amp;t=625\" ><img title=\"Subcellular imaging of organelle dynamics in the early zebrafish brain.\" src=\"https:\/\/www.hhmi.org\/sites\/default\/files\/news\/2018\/movie-5_243352-6_1.gif\" alt=\"\" data-delta=\"15\" data-fid=\"13181\" data-media-element=\"1\" \/><\/a><a href=\"https:\/\/www.youtube.com\/watch?v=VY_v6G_ZLzE&amp;feature=youtu.be&amp;t=625\" >Membrane dynamics in the zebrafish eye.<\/a> <a href=\"https:\/\/www.youtube.com\/watch?v=VY_v6G_ZLzE&amp;feature=youtu.be&amp;t=683\" ><img title=\"Tiled AO correction for imaging large volumes.\" src=\"https:\/\/www.hhmi.org\/sites\/default\/files\/news\/2018\/movie-6_243352-7_1-2.gif\" alt=\"\" data-delta=\"16\" data-fid=\"13182\" data-media-element=\"1\" \/><\/a><a href=\"https:\/\/www.youtube.com\/watch?v=VY_v6G_ZLzE&amp;feature=youtu.be&amp;t=683\" >Tiled AO correction for imaging large volumes.<\/a><\/div>\n<div><a href=\"https:\/\/www.youtube.com\/watch?v=VY_v6G_ZLzE&amp;feature=youtu.be&amp;t=758\" ><img title=\"Organelle dynamics across the zebrafish eye.\" src=\"https:\/\/www.hhmi.org\/sites\/default\/files\/news\/2018\/movie-7_243352-8_1.gif\" alt=\"\" data-delta=\"17\" data-fid=\"13183\" data-media-element=\"1\" \/><\/a><a href=\"https:\/\/www.youtube.com\/watch?v=VY_v6G_ZLzE&amp;feature=youtu.be&amp;t=758\" >Organelle dynamics across the zebrafish eye.<\/a> <a href=\"https:\/\/youtu.be\/VY_v6G_ZLzE?t=909\" ><img title=\"In vivo imaging of spinal cord neural circuit development.\" src=\"https:\/\/www.hhmi.org\/sites\/default\/files\/news\/2018\/movie-8_243352-9_1.gif\" alt=\"\" data-delta=\"18\" data-fid=\"13184\" data-media-element=\"1\" \/><\/a><a href=\"https:\/\/youtu.be\/VY_v6G_ZLzE?t=909\" >In vivo imaging of spinal cord neural circuit development.<\/a> <a href=\"https:\/\/youtu.be\/VY_v6G_ZLzE?t=1052\" ><img title=\"Immune cell migration in the zebrafish inner ear.\" src=\"https:\/\/www.hhmi.org\/sites\/default\/files\/news\/2018\/movie-10_243352-11_1.gif\" alt=\"\" data-delta=\"19\" data-fid=\"13174\" data-media-element=\"1\" \/><\/a><a href=\"https:\/\/youtu.be\/VY_v6G_ZLzE?t=1052\" >Cancer cell migration in a zebrafish xenograft model.<\/a><\/div>\n","protected":false},"excerpt":{"rendered":"<p>HHMI Howard Hughes Medical Institute | An immune cell explores a zebrafish&rsquo;s inner ear By combining two state-of-the-art imaging technologies, Howard Hughes Medical Institute Janelia Research Campus scientists, led by 2014 chemistry Nobel laureate physicist&nbsp;Eric Betzig, have imaged living cells at unprecedented 3D detail and speed, the scientists report on April 19, 2018 in an [&#8230;]<\/p>\n","protected":false},"author":454,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[42,43,44],"tags":[],"class_list":["post-25258","post","type-post","status-publish","format-standard","hentry","category-biotech","category-news","category-physicscosmology"],"_links":{"self":[{"href":"https:\/\/hoo.central12.com\/fugic\/wp-json\/wp\/v2\/posts\/25258"}],"collection":[{"href":"https:\/\/hoo.central12.com\/fugic\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/hoo.central12.com\/fugic\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/hoo.central12.com\/fugic\/wp-json\/wp\/v2\/users\/454"}],"replies":[{"embeddable":true,"href":"https:\/\/hoo.central12.com\/fugic\/wp-json\/wp\/v2\/comments?post=25258"}],"version-history":[{"count":1,"href":"https:\/\/hoo.central12.com\/fugic\/wp-json\/wp\/v2\/posts\/25258\/revisions"}],"predecessor-version":[{"id":25259,"href":"https:\/\/hoo.central12.com\/fugic\/wp-json\/wp\/v2\/posts\/25258\/revisions\/25259"}],"wp:attachment":[{"href":"https:\/\/hoo.central12.com\/fugic\/wp-json\/wp\/v2\/media?parent=25258"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/hoo.central12.com\/fugic\/wp-json\/wp\/v2\/categories?post=25258"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/hoo.central12.com\/fugic\/wp-json\/wp\/v2\/tags?post=25258"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}